Proteinase K (Proteinase K) is a protease with serine as its active center. It has broad substrate specificity, and it preferentially decomposes the ester and peptide bonds of the C-terminus of hydrophobic amino acids, sulfur-containing amino acids and aromatic amino acids.It can degrade proteins in a variety of different salts, denaturants, detergents, pH and temperature conditions.
It is a broad-spectrum RNase inhibitor with a molecular weight of approximately 50 KDa. It can specifically bind to RNase by non-covalent bond to form a complex to inactivate RNase without inhibiting activities of RNase H, nuclease S1, SP6, T7 or T3 RNA polymerase, AMV or M-MLV reverse transcriptase, Taq DNA polymerase and RNase T1. RNasin does not affect subsequent reverse transcription and translation processes. It is widely used in RNA research such as RT-PCR, cDNA synthesis, mRNA protection, in vitro transcription and in vitro translation, preparation of RNase-Free antibodies, in situ hybridization and mRNA localization.
